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WB: 00* IP: 0 IHC: 0** This antibody is effective in immunoblotting, immunoprecipitation, and immunohistochemistry. *Predicted MW = 200-220 k D **Recommend using Bouin's fixative as outlined in the Kamiguchi et al. Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain. H Kamiguchi, KE Long, M Pendergast, AW Schaefer, I Rapoport, T Kirchhausen, V Lemmon. L1CAM, also known as CD171, is a neuronal cell adhesion molecule involved in axon guidance and cell migration with a strong implication in treatment-resistant cancers. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration, and differentiation. PC12 cells utilize the homophilic binding site of L1 for cell-cell adhesion but L1-alphavbeta3 interaction for neurite outgrowth.

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